Inter Vlan Routing

Hello Martyfish​ 

To serve Vlan 40 have F5 as Default Gateway I suppose you also have a Forwarding VS for Vlan 40.
So in this VS you could apply an irule droping traffic form Server1 to Server2

for example

when CLIENT_ACCEPTED {
    set src [IP::client_addr]
    set dst [IP::server_addr]
    
    # Block traffic from 10.10.41.50 to 10.10.10.50
    if { ([IP::addr $src equals 10.10.41.50] and [IP::addr $dst equals 10.10.10.50]) } {
        log local0. "Blocking $src -> $dst"
        reject
    }
}