Persistence and loadbalancing

This is different from our setup where our tier 1 (web) tier is stateful and our tier 2 (web) is stateless, so we don't have this problem, however if you are trying to achieve persistence between the client and the app tier, then unless your web tier is a proxy (in which case it returns cookies from the app tier to the client), then you will need persistence between both client/web and web/app tiers.

FYI if when you say above "I tried the cookie and src persistence in the applica layer", you mean that you have cookie (insert) persistence with source IP fallback persistence, then that means to all intents and purposes that you have source IP persistence (and you nearly always see uneven distribution of load in this case).