SSL handshake failure using serverssl (F5 and Citrix Netscaler)

Hello Anesh / Chase,

Below is the serverssl profile config:

ltm profile server-ssl abc_443_NP_SLG_https_profile_server-ssl {
    alert-timeout 10
    app-service none
    authenticate once
    authenticate-depth 9
    authenticate-name none
    ca-file Comodo_xyz.crt
    cache-size 262144
    cache-timeout 3600
    cert abc.crt
    chain Entrust-EV-256-Bundle-AVX.crt
    ciphers !SSLv2:!SSLv3:!DTLSv1:!EXP:!MD5:!ADH:!NULL:!LOW:!RC4:ECDHE-RSA-AES128-GCM-SHA256:ECDHE-RSA-AES256-GCM-SHA384:AES128-GCM-SHA256:AES256-GCM-SHA384:ECDHE-RSA-AES128-SHA256:ECDHE-RSA-AES256-SHA384:ECDHE-RSA-AES128-CBC-SHA:ECDHE-RSA-AES256-CBC-SHA:AES128-SHA256:AES256-SHA256:AES128-SHA:AES256-SHA:ECDHE-RSA-DES-CBC3-SHA:DES-CBC3-SHA:+TLSv1_1:+TLSv1
    crl-file none
    defaults-from serverssl
    description none
    expire-cert-response-control drop
    generic-alert enabled
    handshake-timeout 10
    key abc.key
    mod-ssl-methods disabled
    mode enabled
    options { dont-insert-empty-fragments }
    partition Common
    passphrase "****"
    peer-cert-mode require
    proxy-ssl disabled
    proxy-ssl-passthrough disabled
    renegotiate-period indefinite
    renegotiate-size indefinite
    renegotiation enabled
    retain-certificate true
    secure-renegotiation require![Image Text](/Portals/0/Users/164/20/128420/SSLFailure.PNG?ver=2018-06-25-001012-333)-strict
    server-name none
    session-mirroring disabled
    session-ticket disabled
    sni-default false
    sni-require false
    ssl-forward-proxy disabled
    ssl-forward-proxy-bypass disabled
    ssl-sign-hash any
    strict-resume disabled
    unclean-shutdown enabled
    untrusted-cert-response-control drop
}

Also, I am attaching a wireshark screenshot of the capture where the SSL handshake fails.