Static route question when the same IP can be either client or server and accessable through outside or inside interface

A few questions: When you say outside and inside interface, do you mean outside and inside vlan? Can you give an example of what the self IP's are on those vlans? When you say in Scenario 2, that the "same ip is accessed via a SNAT through the inside interface" what type of SNAT and what does that configuration look like. Then, the route you're talking about adding? Is that a route for the BIG-IP to learn how to get to the 10.0.0.1 machine?

Making some guesses, if so, the auto-lasthop feature should override your static route so that route should not affect the inbound connection. The BIG-IP will send the packet back to the client the way it came in.