Does updating the Protocol Profile break active connections?

So we are trying to reconfigure lots of our virts to get them all setup the same way with a standard set of options because we currently have lots of various settings based on whoever created them and when it was done. If I change the Protocol Profile (Client and/or Server) for a virt, does that have any affect on anyone actively using the websites? Does it break their connections or anything like that or is it 100% transparent?

 

Just trying to figur o ut if we can work on this during the dya or if it'd be something better left to after hours.

 

Thanks.